Figure 2

Representative flow cytometry comparison of surface NKG2A and intracellular IL-4 co-expression between culture conditions. Flow cytometry comparison of NKG2A and IL-4 co-expression for an exemplary sample of TH2 cells cultured with CD3/CD28 antibodies (positive control) and soluble CD3/CD28/NKG2A antibodies (A). The accompanying histogram shows the similar decrease in overall IL-4 expression in the same positive control (outlined) and Anti-NKG2A samples (shaded) (B). Analyses were gated by forward and side-scatter properties and on CD3+CD4+ cells.