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Table 1 MTT reduction assay in stimulated eosinophils in vitro.

From: Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: Lack of interaction with nitric oxide

Treatment MTT Reduction (% control)
  2 h 3 h 4 h
Eotaxin    
   (10 ng/ml) 84.6 ± 6.6 113.0 ± 6.1 91.0 ± 2.7
   (100 ng/ml) 113.8 ± 5.0* 130.7 ± 2.3* 127.3 ± 3.6*
   (1000 ng/ml) 124.7 ± 5.0* 138.4 ± 2.0* 146.0 ± 11.8*
RANTES    
   (10 ng/ml) 104.8 ± 1.6 128.8 ± 8.0* 105.8 ± 1.0
   (100 ng/ml) 118.9 ± 2.8* 133.4 ± 3.6* 138.4 ± 6.0*
   (1000 ng/ml) 117.4 ± 3.8* 118.0 ± 1.0* 147.0 ± 6.9*
  1. Eosinophil suspension (2 × 106cells/ml) was incubated for 2, 3 or 4 h with eotaxin (10–1000 ng/ml) or RANTES (10–1000 ng/ml). Each experiment was carried out in triplicate (n = 3). Results are expressed as MTT reduction (% control). *P < 0.05 compared to respective untreated cells.