Treatment
|
MTT Reduction (% control)
|
---|
|
2 h
|
3 h
|
4 h
|
---|
Eotaxin
| | | |
(10 ng/ml)
|
84.6 ± 6.6
|
113.0 ± 6.1
|
91.0 ± 2.7
|
(100 ng/ml)
|
113.8 ± 5.0*
|
130.7 ± 2.3*
|
127.3 ± 3.6*
|
(1000 ng/ml)
|
124.7 ± 5.0*
|
138.4 ± 2.0*
|
146.0 ± 11.8*
|
RANTES
| | | |
(10 ng/ml)
|
104.8 ± 1.6
|
128.8 ± 8.0*
|
105.8 ± 1.0
|
(100 ng/ml)
|
118.9 ± 2.8*
|
133.4 ± 3.6*
|
138.4 ± 6.0*
|
(1000 ng/ml)
|
117.4 ± 3.8*
|
118.0 ± 1.0*
|
147.0 ± 6.9*
|
- Eosinophil suspension (2 × 106cells/ml) was incubated for 2, 3 or 4 h with eotaxin (10–1000 ng/ml) or RANTES (10–1000 ng/ml). Each experiment was carried out in triplicate (n = 3). Results are expressed as MTT reduction (% control). *P < 0.05 compared to respective untreated cells.