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Table 1 MTT reduction assay in stimulated eosinophils in vitro.

From: Human eosinophil adhesion and degranulation stimulated with eotaxin and RANTES in vitro: Lack of interaction with nitric oxide

Treatment

MTT Reduction (% control)

 

2 h

3 h

4 h

Eotaxin

   

   (10 ng/ml)

84.6 ± 6.6

113.0 ± 6.1

91.0 ± 2.7

   (100 ng/ml)

113.8 ± 5.0*

130.7 ± 2.3*

127.3 ± 3.6*

   (1000 ng/ml)

124.7 ± 5.0*

138.4 ± 2.0*

146.0 ± 11.8*

RANTES

   

   (10 ng/ml)

104.8 ± 1.6

128.8 ± 8.0*

105.8 ± 1.0

   (100 ng/ml)

118.9 ± 2.8*

133.4 ± 3.6*

138.4 ± 6.0*

   (1000 ng/ml)

117.4 ± 3.8*

118.0 ± 1.0*

147.0 ± 6.9*

  1. Eosinophil suspension (2 × 106cells/ml) was incubated for 2, 3 or 4 h with eotaxin (10–1000 ng/ml) or RANTES (10–1000 ng/ml). Each experiment was carried out in triplicate (n = 3). Results are expressed as MTT reduction (% control). *P < 0.05 compared to respective untreated cells.
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BMC Pulmonary Medicine

ISSN: 1471-2466

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