Figure 3

Histological evaluation and measurement of hydroxyproline. Histological evaluation in representative H&E stained lung sections from vehicle-treated saline mice (Inset in A), vehicle-treated BLM mice (A), and GGA-treated BLM mice (B) on BLM day14. All images at × 400 magnification. (C) Quantitative scoring of the severity of histological lung fibrosis (Ashcroft score). Data shown represent the mean ± SEM from two independent experiments using 5-10 mice for each group. (D) Hydroxyproline assay performed in the lungs from treated mice on BLM day 14. Each group had at least four mice. Data shown represent the mean ± SEM from two independent experiments. *Statistically significant difference (p < 0.05) in comparison. BLM, bleomycin; GGA, geranylgeranylacetone.