Figure 2

Comet assay inin vitroandin vivocells. Comet assay in NCI-H460 cells not exposed to a fluorophore: A/ illumination (2 min.) at 488 nm, B/ illumination (2 min.) at 660 nm. Comet assay in lymphocytes: C/ acriflavine for 2 minutes, no illumination, D/ acriflavine for 2 minutes, then illumination at 488 nm for 2 minutes. Comet assay in lymphocytes incubated with methylene blue: E/ 0.001% (2 min.) without illumination, F/ 0.01% (15 min.) without illumination, G/ 0.001% (2 min.) with illumination at 660 nm (2 min.), H/ 0.01% (15 min.) with illumination at 660 nm (2 min.). Comet Assay in healthy hamster-cheek-pouch cells collected by swab and not put in contact with a fluorophore or illuminated (I).