Objectives, design and enrollment results from the Infant Susceptibility to Pulmonary Infections and Asthma Following RSV Exposure Study (INSPIRE)

Table 3 Specimen Repository for INSPIRE Study

Time Point and Sample Type		*A priori* testing planned
Baseline:
	Infant urine	Markers of inflammation and lung Injury
	Infant nasal filter paper*	Respiratory microbiome, cytokines
	Infant stool sample*	Gut microbiome
	Maternal plasma*	Vitamin E levels
Respiratory Illness:
	Infant urine	Markers of inflammation and lung injury
	Infant nasal wash	PCR identification of RSV or RV; cytokines and biomarkers of immune function; microbiome. RSV positive samples will be sequenced.
One Year:
	Infant blood	RSV serology, DNA banking
	Home doorframe wipe	Endotoxin/Lipopolysaccharide
Two Year Sub-Study:
Child peripheral blood mononuclear cells		Immune response to RSV stimulation
Child nasal wash		Nasal microbiome
Child stool sample		Gut microbiome
Three Year:
Child blood		Serum allergen-specific IgE measurements; if skin prick testing not possible

ISSN: 1471-2466