Fig. 4

SCGB3A2 and proinflammatory cytokine levels in lungs of wild-type (WT) and Scgb3a2-transgenic (TG) mice. a Levels of lung Scgb3a2 mRNAs (left panel) and SCGB3A2 proteins in BALF (right panel) in WT and TG mice at 3, 6, and 9 weeks post-BLM treatment and PBS collected at 3 weeks as control. b Representative immunohistochemistry of SCGB3A2 in WT and TG mice at 3 and 6 weeks post-BLM treatment and PBS collected at 3 weeks as control. Arrow indicates representative SCGB3A2 positive signals found in the airway epithelial cells. Scale bar: 50 μm. c qRT-PCR analysis of lung mRNAs for proinflammatory cytokines, IL-1β, IL-6, and TNFα at 3, 6, and 9 weeks post-BLM treatment and PBS as control. N > 6 in each group. *P < 0.05, **P < 0.01 by student’s t-test