Fig. 2

CT-analysis reveals isolation of fibrosis to specifically treated lung region. CT analysis was performed on one set of lungs ex-vivo to localise the fibrosis and density changes in the lung (a) Siemens Emotion 16 was used (configuration 16x0.6 mm, effective slice. Two reconstructions of the acquired dataset were made: Lung window with edge enhancement filter, Soft tissue window with smoothing filter. (b) Set-up of ex-vivo lung scans: Lungs were floated in a water bath and trachea was secured to a 3 L syringe to inflate the lungs throughout scan. Representative images obtained from CT scan highlighting the isolation of fibrosis to the bleomycin-treated (left caudal) lobe. Images show (c) Volume reformats (Blue 3D) model of the lungs. Areas of air show as hollow surrounded by the blue. Areas of increased density show up as the yellow colour. (d) Slice-image from reconstructed “Lung Thin” dataset, (e) Slice image from reconstructed sagittal reformats