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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Protocadherin-1 is a glucocorticoid-responsive critical regulator of airway epithelial barrier function

Fig. 4

Silencing of PCDH1 does not affect cell growth or apoptosis. 16HBE cells were transfected with control (siCtl) or PCDH1-specific siRNA (siPCDH1_1). Results are mean ± SD. (n = 3). (left) The number of live cells was counted daily for 3 days using trypan blue. (right) Apoptosis was detected by Annexin V/PI staining at day 3 after transfection. The x-axis shows AnnexinV-FITC binding and the y-axis pertains to the results for staining with the vital dye propidium iodide. Cells in the lower left quadrant are viable, cells in the lower right are apoptotic, and those in the upper right are late stage apoptotic/dead cells

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BMC Pulmonary Medicine

ISSN: 1471-2466

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