Fig. 7
From: Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge
Immunostaining of IL-4 and IL-13 in lung tissues following allergen challenge, showing (a) negative staining (isotype-matched control Ab), (b) IL-4+ cells (red, arrows), (c) CD45+ leukocyte (red) staining, and (d) CD45+ (red) and IL-13+ (green, arrows) staining; the arrow shows an IL-13 expressing leukocyte (IL-13+CD45+) and the arrow head shows an IL-13 expressing non-leukocyte (IL-13+CD45−). Representative sections were taken from the same lung lobe of a sheep, collected post-mortem at 48 h post-HDM challenge. All slides were counterstained with DAPI (blue) when mounted in Mowiol (Original magnification × 400)