Table 1 Details of reagents used in ovine-specific cytokine ELISAs
From: Dynamics of IL-4 and IL-13 expression in the airways of sheep following allergen challenge
ELISA | Antibody (Ab)/cytokine details | Clone/type | Dilution | Source |
|---|---|---|---|---|
IL-4 | rov IL-4 (cytokine standard) | - | - | Moreduna |
Anti-bovine IL-4 mAb (coating) | CC313 | 1:400 | AbD Serotecb | |
Anti-bovine IL-4/biotinylated mAb (detecting) | CC314 | 1:1600 | AbD Serotec | |
Streptavidin/HRP | - | 1:1000 | Dakoc | |
IL-6 | rov IL-6 (cytokine standard) | - | - | CABd |
Anti-ovine IL-6 mAb (coating) | 4B6 | 1:400 | Epitopee | |
Anti-ovine IL-6, pAb (detecting) | rabbit pAb | 1:5000 | CAB | |
Anti-rabbit Ig/HRP | - | 1:1000 | Dako | |
IL-10 | rbov IL-10Â (cytokine standard) | - | - | Moredun |
Anti-bovine IL-10 mAb (coating) | CC318 | 1:200 | AbD Serotec | |
Anti-bovine IL-10/biotinylated mAb (detecting) | CC320 | 1:1000 | AbD Serotec | |
Streptavidin-HRP | - | 1:1000 | Dako | |
IL-13 | rbov IL-13 (cytokine standard) | - | - | Kingfisherf |
Anti-bovine IL-13, pAb (coating) | rabbit pAb | 1:800 | Kingfisher | |
Anti-bovine IL-13/biotinylated pAb (detecting) | rabbit pAb | 1:2000 | Kingfisher | |
Streptavidin/HRP | - | 1:1000 | Dako | |
TNFα | rov TNFα (cytokine standard) | - | - | CAB |
Anti-ovine TNFα mAb ascites (coating) | 6.09 | 1:200 | CAB | |
Anti-ovine TNFα pAb (detecting) | rabbit pAb | 1:500 | CAB | |
Anti-rabbit Ig/HRP | - | 1:1000 | Dako |