Fig. 2

Image SXM and ImageJ methodology. Image SXM (a, b & c); digital images of the cytospins (a) were manually edited to remove all non-macrophage cells and debris (b). Image SXM then calculated the area of cytoplasm [27] and particulate matter (red), mapped out in the output image (c). ImageJ (d, e & f): for each macropghage, the threshold level was adjusted manually until the black areas of particulate matter seen in the original image (a) turned red (b). The particulate matter within the cytoplasm was then selected by freehand (c)