Fig. 3
From: Role of CD248 as a potential severity marker in idiopathic pulmonary fibrosis
Measurement of expression levels of CD248 on human lung fibroblasts derived from normal (n = 6) and IPF lungs (n = 6). Panel a: Flow cytometric histograms showing CD248 expression on human lung fibroblasts from normal (diagram on the left) and IPF lungs (diagram on the right). Open histograms represent samples labelled with FITC-conjugated CD248 antibody, closed histograms represent FITC-conjugated isotype control antibody. Two representative diagrams are shown of twelve independent samples. Panel b: CD248 expression changes in fibroblasts treated with TGF-beta (n = 12). Bars represent median fluorescent intensities (average + SEM is shown of six independent samples). CD248 expression levels were significantly higher (p < 0.001) in fibroblasts derived from IPF patients than in those from normal lungs. CD248 expression declined significantly (p < 0.01) upon TGF-beta treatment in fibroblasts derived from IPF lungs but not in those from normal lungs. Panel c: CD248 mRNA expression levels in fibroblasts treated with TGF-β1 derived from normal (n = 6) and IPF lungs (n = 6). Bars repesent CD248 Ct values compared to GAPDH. Note that there are no significant differences between CD248 mRNA expression in different samples