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Fig. 5 | BMC Pulmonary Medicine

Fig. 5

From: Baicalin attenuates bleomycin-induced pulmonary fibrosis via adenosine A2a receptor related TGF-β1-induced ERK1/2 signaling pathway

Fig. 5

A2aR and baicalin attenuated ERK1/2 phosphorylation in the BLM mouse model. a Images of p-ERK1/2 immunohistochemistry in lung tissue sections. Magnification ×400, scale bars represent 100 μm. b Quantitative analysis of p-ERK1/2 protein by immunohistochemistry. c The protein expression ratios of phospho-ERK1/2 to total-ERK1/2. d Images of p-ERK1/2 in lung homogenate (western blot). e Quantitative analysis of p-ERK1/2 in lung by western blot. f Images of total-ERK1/2 in lung homogenate (western blot). g Quantitative analysis of total-ERK1/2 in lung by western blot. GAPDH was used as internal control (n = 3). Data were expressed as mean ± SD. ** p < 0.01 vs control group, ## p < 0.01 vs model group, # p < 0.05 vs model group, ++ p < 0.01 between WT and KO groups, + p < 0.05 between WT and KO groups

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