Fig. 2

CD11b expression on neutrophils after their incubation with serum samples from major trauma patients (TP). Serum samples were obtained from TP (n = 18, all). TP were grouped to no inf. without pneumonia (n = 9) or inf. group with pneumonia (n = 9). Samples were obtained at admission to emergency department (ED), a day prior pneumonia diagnosis (1 d prior inf) or at the day of diagnosis (1 d prior inf). Samples from the equal post-injury days in the corresponding no inf. group were used. Neutrophils were isolated from healthy volunteers (HV, n = 9). CD11b expression on neutrophils after their incubation with TP’s serum (20% in culture medium) or control without serum addition (ctrl) for two hours was determined by flow cytometry. Data are represented as mean ± SEM. p < 0.05 *: vs. ctrl, #: no inf. vs. corresponding inf. group