Fig. 9
From: NOX4 expression and distal arteriolar remodeling correlate with pulmonary hypertension in COPD
Impacts of TGFβ1 on the production of Collagen type 1 alpha1, malondialdehyde (MDA) and superoxide dismutase (SOD) in human primary artery smooth muscle cells (HPASMCs). HPASMCs were cultured in the presence of TGFβ1 at various concentrations (0–10 ng/mL) for different time periods (0–48 h) before the supernatants were used for analysis. a The production of Collagen type 1 alpha1 in HPASMCs exposed to 2.0 ng/ml of TGF-β1 for indicated time (left panel) and to indicated concentration of TGFβ1 for 24 h (right panel); (b) A higher concentration of MDA (left panel) but a lower concentration of SOD (right panel) in sera of COPD patients relative to healthy subjects; (c) the production of MDA (left panel) and SOD (right panel) in HPASMCs exposed to 2.0 ng/ml of TGF-β1 for indicated time; (d) the production of MDA (left panel) and SOD (right panel) in HPASMCs exposed to indicated concentration of TGFβ1 for 24 h. Compared to untreated group, *: P < 0.05. Bar graphs represent mean ± SEM from three independent experiments. Compared to untreated group, *: P < 0.05; **: P < 0.01; ***: P < 0.001