Fig. 1

WISP1 is Highly Increased in Lung Tissue Following Hepatic IRI. qRT-PCR (a) and western blot (b) analysis of WISP1 in lung samples from WT mice undergoing ischemia for 60 min and reperfusion (I/R) for 0 h, 6 h, 12 h, and 24 h (n = 6 respectively). c Immunofluorescence and (d) Immunohistochemistry assays of WISP1 in lung samples from WT mice in sham and IRI group undergoing ischemia for 60 min and reperfusion for 6 h. Gene expression was normalized to β-actin mRNA levels in each sample. ^*p < 0.05 compared with 0 h of reperfusion, data are expressed as mean ± s.e.m. for all samples