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Table 1 Expression of selected angiogenic genes in the lung by qRT-PCR in newborn mice exposed to hyperoxia (95%O2) or room air and administered caffeine or saline

From: Caffeine is associated with improved alveolarization and angiogenesis in male mice following hyperoxia induced lung injury

Gene Symbol

Room Air-Caffeine (RAC) Group

Hyperoxia-Saline (HS) Group

Hyperoxia-Caffeine (HC) Group

Hif-1α

2.28 ± 1.05

2.31 ± 0.33

2.35 ± 0.85

Hif-2α

2.62 ± 0.87*

2.67 ± 0.44*

1.52 ± 0.70

Hif-3α

2.83 ± 1.25

1.72 ± 0.44

1.64 ± 0.71

Hif-1β

1.77 ± 0.34

1.65 ± 0.20

1.51 ± 0.43

PHD-2

1.78 ± 0.30*

1.89 ± 0.46*

1.00 ± 0.26

VEGF

2.00 ± 0.42*

3.52 ± 0.66*

1.17 ± 0.34

Flt-1

2.71 ± 0.89

2.44 ± 0.40

1.93 ± 0.57

Ang-1

2.85 ± 1.26*

1.89 ± 1.11

0.89 ± 0.27

  1. The fold-change (FC) in gene expression is compared to the room air-saline (RAS) group (control group; FC in RAS = 1.0. (n = 8 pups in each group)
  2. Values expressed as fold change ± SD compared to room air saline group. *p < 0.05 versus hyperoxia-caffeine group (Fisher’s post-hoc test, ANOVA); Hif-1α - Hypoxia-Inducible Factor-1α; HIF-2α - Hypoxia-Inducible Factor-2α; HIF-3α - Hypoxia-Inducible Factor-3α; Hif-1β - Hypoxia Inducible Factor-1β; PHD-2: Prolyl Hydroxylase – 2 (EGLN1); VEGF - Vascular Endothelial Growth Factor; Flt-1: VEGF Receptor 1; Ang-1: Angiopoietin-1. Housekeeping genes – Phosphoglycerate kinase-1 (Pgk1); Peptidylprolyl isomerase A (Ppia)
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BMC Pulmonary Medicine

ISSN: 1471-2466

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