|
Gene Symbol
|
Room Air-Caffeine (RAC) Group
|
Hyperoxia-Saline (HS) Group
|
Hyperoxia-Caffeine (HC) Group
|
|---|
|
Hif-1α
|
2.28 ± 1.05
|
2.31 ± 0.33
|
2.35 ± 0.85
|
|
Hif-2α
|
2.62 ± 0.87*
|
2.67 ± 0.44*
|
1.52 ± 0.70
|
|
Hif-3α
|
2.83 ± 1.25
|
1.72 ± 0.44
|
1.64 ± 0.71
|
|
Hif-1β
|
1.77 ± 0.34
|
1.65 ± 0.20
|
1.51 ± 0.43
|
|
PHD-2
|
1.78 ± 0.30*
|
1.89 ± 0.46*
|
1.00 ± 0.26
|
|
VEGF
|
2.00 ± 0.42*
|
3.52 ± 0.66*
|
1.17 ± 0.34
|
|
Flt-1
|
2.71 ± 0.89
|
2.44 ± 0.40
|
1.93 ± 0.57
|
|
Ang-1
|
2.85 ± 1.26*
|
1.89 ± 1.11
|
0.89 ± 0.27
|
- The fold-change (FC) in gene expression is compared to the room air-saline (RAS) group (control group; FC in RAS = 1.0. (n = 8 pups in each group)
- Values expressed as fold change ± SD compared to room air saline group. *p < 0.05 versus hyperoxia-caffeine group (Fisher’s post-hoc test, ANOVA); Hif-1α - Hypoxia-Inducible Factor-1α; HIF-2α - Hypoxia-Inducible Factor-2α; HIF-3α - Hypoxia-Inducible Factor-3α; Hif-1β - Hypoxia Inducible Factor-1β; PHD-2: Prolyl Hydroxylase – 2 (EGLN1); VEGF - Vascular Endothelial Growth Factor; Flt-1: VEGF Receptor 1; Ang-1: Angiopoietin-1. Housekeeping genes – Phosphoglycerate kinase-1 (Pgk1); Peptidylprolyl isomerase A (Ppia)
