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Table 1 Expression of selected angiogenic genes in the lung by qRT-PCR in newborn mice exposed to hyperoxia (95%O2) or room air and administered caffeine or saline

From: Caffeine is associated with improved alveolarization and angiogenesis in male mice following hyperoxia induced lung injury

Gene Symbol Room Air-Caffeine (RAC) Group Hyperoxia-Saline (HS) Group Hyperoxia-Caffeine (HC) Group
Hif-1α 2.28 ± 1.05 2.31 ± 0.33 2.35 ± 0.85
Hif-2α 2.62 ± 0.87* 2.67 ± 0.44* 1.52 ± 0.70
Hif-3α 2.83 ± 1.25 1.72 ± 0.44 1.64 ± 0.71
Hif-1β 1.77 ± 0.34 1.65 ± 0.20 1.51 ± 0.43
PHD-2 1.78 ± 0.30* 1.89 ± 0.46* 1.00 ± 0.26
VEGF 2.00 ± 0.42* 3.52 ± 0.66* 1.17 ± 0.34
Flt-1 2.71 ± 0.89 2.44 ± 0.40 1.93 ± 0.57
Ang-1 2.85 ± 1.26* 1.89 ± 1.11 0.89 ± 0.27
  1. The fold-change (FC) in gene expression is compared to the room air-saline (RAS) group (control group; FC in RAS = 1.0. (n = 8 pups in each group)
  2. Values expressed as fold change ± SD compared to room air saline group. *p < 0.05 versus hyperoxia-caffeine group (Fisher’s post-hoc test, ANOVA); Hif-1α - Hypoxia-Inducible Factor-1α; HIF-2α - Hypoxia-Inducible Factor-2α; HIF-3α - Hypoxia-Inducible Factor-3α; Hif-1β - Hypoxia Inducible Factor-1β; PHD-2: Prolyl Hydroxylase – 2 (EGLN1); VEGF - Vascular Endothelial Growth Factor; Flt-1: VEGF Receptor 1; Ang-1: Angiopoietin-1. Housekeeping genes – Phosphoglycerate kinase-1 (Pgk1); Peptidylprolyl isomerase A (Ppia)
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