Fig. 1

HDM induced the expression of secreted Hsp90α in asthmatic mice and the effect of 1G6-D7 on animal models. a: Lung sections were stained with H&E.Magnification,200 (top panel) and 400 (bottom panel).b: Airway resistance (RL) was assessed 24 h after the last administration. Exponentially increasing concentration of methacholine caused higher RL in the HDM group than in con group, mice pretreated with 1G6-D7 showed a partly reduction of RL. Data shown are means±SD, (*P < 0.05 vs the control group; ^#P < 0.05 vs the HDM group). c: Immunohistochemical staining of HSP90α (brown) in serial lung sections of asthmatic mice. Magnification,200 (top panel) and 400 (bottom panel). d&e: The secretion of Hsp90α in BALF and serum samples from mice (n = 8) was assessed by ELISA. Data shown are means±SD, (*P < 0.05 vs the control group;* ^#P < 0.05 vs the HDM group)