Fig. 1

The evaluation of the animal model establishment. a HE staining was used to detect the pathological changes in lung tissues (× 200, n = 8). b Lung function parameters including FEV0.1, Cydn and Raw were evaluated. c Inflammatory cytokines such as IL-6, TNF-α and IL-10 in BALF were detected using ELISA (n = 8). d Oxidative stress related cytokines including MDA, SOD and GSH were evaluated using ELISA assay (n = 8). e Cell counts of total cell, mononuclear cell and neutrophils in BALF were carried out (n = 8). f. IHC was performed to evaluate the expression of ki67 in lung tissue (× 100, n = 8). *p < 0.05, **p < 0.01. Three independent biological repeats were carried out for each analysis