Fig. 4

Autophagy-associated protein and mRNA expression in m. gastrocnemius in response to IT-LPS. mRNA expression levels of Lc3b (b), Gabarapl1 (c) and sqstm1 (d) in m. gastrocnemius are depicted. Protein levels of LC3B (e-g), GABARAPL1 (h) and SQSTM1 (i) in m. gastrocnemius are depicted. Representative immunoblots and a representative part of Ponceau S staining are shown, with cropped photographs indicated by black boxes, with adjusted contrast equally applied to whole photograph (a). For LC3B and SQSTM1, samples were grouped by time-point over different gels which were corrected for loading controls. For GABARAPL1, all samples were equally divided over multiple gels which were derived and processed in parallel. Data is presented as box plots indicating median and interquartile range, with whiskers indicating min and max. p-value and significance is depicted between groups within each time-point *p < 0.05