Fig. 3

a Pearson correlation coefficients (r²) of the microarray data between two samples were calculated using the average (log₂-transformed) gene expression values. b Representative flow cytometry plot showing the expression of Sca-1 on lin^negPDGFRA^high cells (see Fig. 1a). Sca-1^high fibroblasts and Sca-1^low fibroblasts are indicated. Quantitative PCR for (c) collagen 1a1 (Col1a1), Cd248, decorin (Dcn), nephronectin (Npnt), elastin (Eln), lecithin-retinol acyltransferase (Lrat), thrombospondin (Thbs1), and syndecan 4 (Sdc4) were performed using prepared cDNA samples of the freshly isolated Sca-1^high and Sca-1^low fibroblasts, measured relative to the level of glyceraldehyde-3-phosphate dehydrogenase (Gapdh) for each sample, which was adjusted to 100. Three independent experiments were performed in triplicate. Mean values ± standard deviations are presented; **P < 0.01. d Upper: Quantitative PCR for integrin alpha-8 (Itga8). Lower left: Representative flow cytometry plot showing the expression of ITGA8 on Sca-1^high fibroblasts (blue line) and Sca-1^low fibroblasts (red line). Lower right: Quantitative analysis of ITGA8 expression by FACS. Isotype indicates flow cytometric analysis using biotin-conjugated goat antibody as a control. Three independent experiments were performed in triplicate. Mean values ± standard deviations are presented; **P < 0.01