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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: CD248 and integrin alpha-8 are candidate markers for differentiating lung fibroblast subtypes

Fig. 3

a Pearson correlation coefficients (r2) of the microarray data between two samples were calculated using the average (log2-transformed) gene expression values. b Representative flow cytometry plot showing the expression of Sca-1 on linnegPDGFRAhigh cells (see Fig. 1a). Sca-1high fibroblasts and Sca-1low fibroblasts are indicated. Quantitative PCR for (c) collagen 1a1 (Col1a1), Cd248, decorin (Dcn), nephronectin (Npnt), elastin (Eln), lecithin-retinol acyltransferase (Lrat), thrombospondin (Thbs1), and syndecan 4 (Sdc4) were performed using prepared cDNA samples of the freshly isolated Sca-1high and Sca-1low fibroblasts, measured relative to the level of glyceraldehyde-3-phosphate dehydrogenase (Gapdh) for each sample, which was adjusted to 100. Three independent experiments were performed in triplicate. Mean values ± standard deviations are presented; **P < 0.01. d Upper: Quantitative PCR for integrin alpha-8 (Itga8). Lower left: Representative flow cytometry plot showing the expression of ITGA8 on Sca-1high fibroblasts (blue line) and Sca-1low fibroblasts (red line). Lower right: Quantitative analysis of ITGA8 expression by FACS. Isotype indicates flow cytometric analysis using biotin-conjugated goat antibody as a control. Three independent experiments were performed in triplicate. Mean values ± standard deviations are presented; **P < 0.01

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