Fig. 2

Phylogenetic analysis of six tumor lesions. Mutational profiling using whole-exome sequencing revealed that L4 and L6 shared a total of 28 nonsynonymous mutations. L1, L2, and L3 shared three nonsynonymous mutations, including the KRAS G12C hotspot mutation. L5 represented an independent lesion of distinct lineage. The numbers of nonsynonymous mutations detected in different lesions are indicated with the representative mutations shown. Chr1q gain was detected in all lesions. The colors indicate the chr1q gain as either paternal or maternal based on the analysis of the alternate allelic frequencies of heterozygous SNPs (refer to Figure S1 for additional details)