Fig. 2

Effects of hyperoxia on the expressions of p-SPAK and claudin-18 of alveolar epithelium in hyperoxic acute lung injury. a Representative images of immunofluorescence staining for p-SPAK (TRITC-labeled red) and claudin-18 (FITC-labeled green) of mouse lungs (original magnification × 600). Nuclei were counterstained with DAPI (blue). b p-SPAK expressions in mouse lungs determined by western blot analysis (n = 5). c Claudin-18 expressions in mouse lungs determined by western blot analysis (n = 6). WT: wild type. SPAK-KO: SPAK knock-out. p-SPAK: phosphorylated-SPAK. Data are expressed as the means ± SD. *p < 0.05 versus normoxia WT; ^#p < 0.05 versus hyperoxia WT