Fig. 3

Effects of ROS on the expressions of p-SPAK in MLE-12 cells exposed to hyperoxia. a Effects of hyperoxia duration on p-SPAK expressions determined by western blot analysis (n = 4). *p < 0.05 versus 0 h. b Effects of n-acetylcysteine pretreatment on p-SPAK expressions in MLE-12 cells exposed to hyperoxia determined by western blot analysis (n = 3). *p < 0.05 versus normoxia control; ^#p < 0.05 versus hyperoxia control. c Effects of ROS-generating enzyme inhibitors on p-SPAK expressions detected by in-cell western assay and the quantitative values from intensity of p-SPAK (n = 3). *p < 0.05 versus control. d Effects of indomethacin 5 μM on p-SPAK expressions determined by western blot analysis (n = 3). *p < 0.05 versus normoxia control; ^#p < 0.05 versus hyperoxia control. WT wild type, NAC n-acetylcysteine, H.C. high concentration, L.C. low concentration, p-SPAK phosphorylated-SPAK. Data are expressed as the mean ± SD