Fig. 6

POU2F2 promotes the proliferation, migration, and invasion of lung cancer cells via targeting AGO1. a Immunoblot assays showed the expression level of AGO1 in both A549 and H1299 cells transfected with vector or plv-AGO1 plasmids. These images were analyzed with the Image J (National Institutes of Health, USA). Original image blots are presented in Additional file 7. b CCK-8 assays were conducted to detect the proliferation capacity of A549 and H1299 cells upon the transfection of the indicated siRNA or plasmids, and the OD value at 490 nm wavelength was detected. c Colony formation assays were conducted to detect the proliferation capacity of A549 and H1299 cells upon the transfection of the indicated siRNA or plasmids, and the colony number was shown. d Transwell migration assays were conducted to detect the migration degree of A549 and H1299 cells upon the transfection of the indicated siRNA or plasmids, and the OD value at 490 nm wavelength was detected. e Transwell invation assays were conducted to detect the invasion degree of both A549 and H1299 cells upon the transfection of the indicated siRNA or plasmids, and the OD value at 490 nm wavelength was measured. Results are presented as mean ± SEM, *p < 0.05, **p < 0.01, ^##p < 0.001