Fig. 4

GATA6-AS1 mediated the proliferation, migration, invasion, apoptosis of LUAD cells by GATA6. a The RT-qPCR analysis measured the knockdown efficiency of GATA6 in A549 and H1975 cells. b, c The EdU assay analyzed the influence of GATA6 silence on proliferative rate of A549 and H1975 cells reduced by overexpressed GATA6-AS1. d The proliferative cells decreased by GATA6-AS1 upregulation was affected by depleting GATA6, as suggested by the colony formation assay. e The TUNEL assay was conducted to examine how GATA6-AS1 overexpression-suppressed cell apoptosis was neutralized after downregulated GATA6. f Flow cytometry analysis investigated the change of apoptotic rate in A549 and H1975 cells after transfected with upregulated GATA6-AS1 and then upregulated GATA6-AS1 plus silencing GATA6. g–h The effects of GATA6 knockdown on GATA6-AS1 upregulation-repressed cell migration and invasion were measured utilizing the wound healing and transwell assays. **p < 0.01