Fig. 6

Effect of oncogenic EML4-ALK tyrosine kinase on the biological behaviors of lung cancer cells. a EML4-ALK levels in transfected H2228 and HEK293 cells. b Increased apoptosis in H2228 cells following EML4-ALK knockdown measured by flow cytometry. c Cell viability, measured by the CCK8 assay, was increased by EML4-ALK expression in HEK293 cells. d–e Viability and proliferation of H2228 cells decreased after knockdown of EML4-ALK or STAT6. f H2228 cell viability following treatment with ALK or JAK-STAT pathway inhibitors as measured by the CCK-8 assay. *P < 0.05