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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Long non-coding RNA OIP5-AS1 aggravates acute lung injury by promoting inflammation and cell apoptosis via regulating the miR-26a-5p/TLR4 axis

Fig. 2

TLR4 was upregulated by LPS stimulation and negatively modulated by miR-26a-5p in PBECs. A RNA22 v2 database predicted the binding sequences of miR-26a-5p and TLR4. B RT-qPCR was conducted to assess the efficiency of miR-26a-5p overexpression and miR-26a-5p knockdown in PBECs. C Luciferase reporter assay was performed to verify the interaction between miR-26a-5p and TLR4 in PBECs. D, E RT-qPCR and western blot assay were applied for detecting the effects of miR-26a-5p overexpression and miR-26a-5p knockdown on the mRNA and protein expression of TLR4 in PBECs. F, G The expression of miR-26a-5p in mice’s lung tissues (n = 8 per group) and LPS-treated PBECs was assessed by RT-qPCR analysis. #P < 0.05 compared with Mock group in B–E; #P < 0.05 compared with Sham group in F; #P < 0.05 compared with control group in G

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BMC Pulmonary Medicine

ISSN: 1471-2466

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