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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: Circular RNA NEK6 contributes to the development of non-small-cell lung cancer by competitively binding with miR-382-5p to elevate BCAS2 expression at post-transcriptional level

Fig. 1

Circ_NEK6 plays a carcinogenic role in NSCLC. A Relative expression of circ_NEK6 in 70 pairs of NSCLC samples was examined, and circ_NEK6 expression was detected by RT-qPCR in normal 16HBE cells and NSCLC cells (including NCI-H23, PC-9, A-549 and NCI-H1869). B Interference efficiency of shRNAs targeting circ_NEK6 was assessed via RT-qPCR assay. C, D Colony formation and EdU assays were conducted to uncovered the effect of circ_NEK6 down-regulation on cell proliferation. E, F The apoptosis abilities of NSCLC cells were assessed by JC-1 assay and flow cytometry analysis. G Transwell assay was performed to detect the migration of NSCLC cells. H Western blot was conducted to analyze the protein level of MMP2 and MMP9 proteins in cells with silenced circ_NEK6 or not. I The capacity of cell invasion was detected by Transwell assay. All results were displayed as the mean ± SD. *P < 0.05, **P < 0.01

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BMC Pulmonary Medicine

ISSN: 1471-2466

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