Fig. 4

DLGAP1-AS2 overexpression increased the expression of cyclin D1, a target of miR-503. To test possibility that DLGAP1-AS2 acts as an endogenous sponge of miR-503, the effects of DLGAP1-AS2 and miR-503 overexpression on cyclin D1 expression were explored by RT-qPCR (A) and Western blot (B). Mean ± SD values of three biological replicates were used to express data of multiple transfection groups. *p < 0.05