Fig. 2
From: LncRNA MANCR positively affects the malignant progression of lung adenocarcinoma
Downregulating MANCR hampers LUAD cell proliferation while promoting cell apoptosis. Cell lines with silenced MANCR were examined. A Expression of MANCR in cells transfected with sh-MANCR. B Cell viability was tested using the CCK-8 assay. C Colony formation assay was used to test the cell proliferative ability. D Flow cytometry detected cell cycle changes after transfection with sh-MANCR. E Western blot detected the expression of cyclins Cyclin D1, CDK2, and CDK4 and proliferation-associated protein PCNA in cell lines. F Flow cytometry detected cell apoptosis after transfection. G Western blot detected the expression of apoptosis-related proteins; *p < 0.05, **p < 0.01, n = 3