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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: LncRNA LINC00520 aggravates cell proliferation and migration in lung adenocarcinoma via a positive feedback loop

Fig. 3

LINC00520 upregulated FOXP3 expression by binding to miR-3611. a Effect of sh-LINC00520#1/2 on FOXP3 expression was testified by RT-qPCR. b The overexpression efficiency of LINC00520 and the impact of LINC00520 upregulation on FOXP3 expression in LUAD cells were determined by RT-qPCR. c Subcellular localization of LINC00520 was confirmed with subcellular fractionation assay in LUAD cells. d Venn pattern showed three putative shared miRNAs for LINC00520 and FOXP3 with online tool DIANA and miRDB. e RT-qPCR was conducted to detect expression of 3 putative miRNAs in LUAD cells. f MiR-3611 levels in LUAD tissues and adjacent non-tumor tissues was determined via RT-qPCR. g The overexpression efficiency of miR-3611 in LUAD cells was tested through RT-qPCR analysis. h Binding sequence between miR-3611 and LIN00520 (FOXP3) were predicted through DIANA and miRDB, respectively. i Luciferase reporter experiment verified binding ability of miR-3611 and FOXP3. j Enrichment of miR-3611, LINC00520, FOXP3 precipitated by Ago2 was further validated by RIP assay. ***p < 0.001

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BMC Pulmonary Medicine

ISSN: 1471-2466

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