Fig. 2

Experimental protocol used to establish the CH model and survival study. A, CH model: Pulmonary and carotid arteries were catheterized on the final day of chronic hypoxia (CH) exposure; awake mean pulmonary artery pressure (mPAP) and mean artery pressure (mAP) were measured on day 15 with rats fully awake; lung and heart samples were obtained for measurements of the right ventricle weight-to-left ventricle + septum weight ratio (RV/LV + S), morphometry of pulmonary arteries, Western blotting and PCR after the pressure measurements. Air/CLZ-, rats exposed to ambient air and fed rat chow without CLZ; Air/CLZ +, rats exposed to ambient air and fed rat chow with CLZ; CH/CLZ-, rats exposed to chronic hypoxia and fed rat chow without CLZ; CH/CLZ +, rats exposed to chronic hypoxia and fed rat chow with CLZ. (n) = number of rats used. The number of rats used (n) was not always the same as the numbers listed in Figs. 5, 6 and 10. See the legend of Fig. 1. B, Survival experiment. MCT/CLZ-, rats injected with MCT and fed rat chow without CLZ. MCT/CLZ +, rats injected with MCT and fed rat chow with CLZ. MCT, monocrotaline; (n) = number of rats used