Fig. 5

mRNA expression levels of a interleukin (IL)-6, b IL-4, c IL-13, d IL-10, e fibronectin and f type 1 collagen (COL1) were measured using SYBR Green 2-step real-time reverse transcriptase polymerase chain reaction (RT-PCR). Inhaling hydrogen gas significantly suppressed the expression of IL-6, IL-4, and IL-13 induced by bleomycin administration. The expression of fibronectin is significantly reduced by hydrogen inhalation. SA, saline administration and air inhalation, n = 12; SH, saline administration and hydrogen inhalation, n = 12; BA, bleomycin administration and air inhalation, n = 28, BH, bleomycin administration and hydrogen inhalation, n = 28. NS: not significant, *p < 0.05, **p < 0.01. Error bars: 95% CI