Fig. 6

IL-8 is a potential mediator of HB-EGF-BEAS-2B-CM and HB-EGF-HBE-CM in fibroblasts activation. The CCK-8 assay assessed the proliferation of fibroblasts. IL-8 of CM was depleted by antibody (ab). Lung fibroblasts were stimulated with depletion IL-8 CM of BEAS-2B (a) and HBECs (b) for 24 h. Lung fibroblast migration was measured by transwell migration assay. HB-EGF-BEAS-2B-CM + IL-8 antibody (c, e) and HB-EGF-HBE-CM + IL-8 antibody (d, f) was used to culture fibroblasts for 24 h. Data are from one experiment representative of three independent experiments. Results are expressed as mean ± SEM. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^###P < 0.0001 versus control medium of BEAS-2B cells; *P < 0.05, **P < 0.01, ***P < 0.001 versus control medium of HBECs