Fig. 6
Knockdown of miR-942-5p modulates the functional behaviors and gefitinib sensitivity of A549/GR and PC9/GR cells by up-regulating TGFBR2. A549/GR and PC9/GR cells were transfected with anti-miR-NC, anti-miR-942-5p, anti-miR-942-5p + si-NC, or anti-miR-942-5p + si-TGFBR2 and checked for TGFBR2 mRNA expression by qRT-PCR analysis (A), TGFBR2 protein level by western blot (B), IC50 value for gefitinib by MTT assay (C), cell proliferation by EdU assay (D), cell colony formation by colony formation assay (E), cell proliferation by MTT assay (F, G), cell apoptosis by flow cytometry (H), cell migration and invasion by transwell assay (I, J), Twist1 and E-cadherin levels by western blot (K, L). *P < 0.05