Fig. 4

Effects of MG132 treatment on ubiquitin proteasome pathway in AECII during hyperoxia exposure. After MG132 treatment with varying concentration (0, 5, 10, 15 and 50 μmol/L), cell viability was detected by MTT assay (A) and the activity of 20 s proteasome was detected by Reagent kit with specific fluorescent substrate (B). Compared with Control group and DMSO group, MG132 group (MG132 concentration of 10 μmol/L) showed a decreased activity of 20 s proteasome (C) and an increased expression of total ubiquitinated proteins (D). The grouping of blots cropped from different parts of the same gel and β-actin was used as the loading control. The part of original data of D were presented in Additional file 1: Fig. S4. Values represent mean ± SD; ** P < 0.05 versus control