Fig. 4

Representative Tunel images of apoptosis (magnification × 200 and × 400): The apoptosis rate is calculated by taking 4 visual fields per slice and measuring the percentage of apoptotic cells in 100 cells in each visual field, The number of apoptotic cells in H group increased significantly, while that in T group decreased significantly compared with H group (a, b); Representative IHC images of Fasl (magnification × 200 and × 400) Microscopically, Fasl protein was mainly expressed in the cytoplasm, after the intervention of T-5224, the expression of caspase3 decreased significantly (c); levels of caspase3 protein in Lung tissue, after the intervention of T-5224, the expression of caspase3 decreased significantly (d, e). C: Control Group, Tidal volume (TV): 0; H: High tidal volume group, TV: 30 ml/kg; D: DMSO group, TV: 30 ml/kg, Half an hour before making the model, the DMSO was injected; T: T-5224 group, TV: 30 ml/kg, Half an hour before making the model, the T-5224 was injected, T1 (0.3 mg/kg), T2 (3 mg/kg). Data are expressed as the mean ± SD (n = 6 Rat/group). ****P < 0.0001 versus C;**P < 0.01 versus C; *P < 0.05 versus C; ####P < 0.0001 versus H; ###P < 0.001 versus H; #P < 0.05 versus H