Fig. 2

Differentially expressed genes (DEGs) identified in C1 and C3, or C2 and C3 subtypes of LUAD. A Volcano map of DEGs for C1 and C3 subtypes. DEGs were obtained by the DESeq2 algorithm (false discovery rate ≤ 0.05 and log2 fc > 1.5). Red dots indicate upregulated genes and blue dots indicate downregulated genes (left). Functional and pathway analyses were performed using the R-package clusterProfiler module (right). B Enrichment analysis of DEGs in C2 and C3 subtypes. C The mutation load among different immune subtypes in LUAD as analyzed by the Chi-square test. Rows represent genes, and columns represent samples. The intersection represents the sample mutation, the color represents the mutation type, and the color at the bottom represents immune typing. D Analysis of differences in FLG, MUC17, and p53 mutations among different subtypes of LUAD