Fig. 6

ZIP12 promoted the proliferation and migration of PASMCs via the AKT/ERK signaling pathways. Ctrl-PASMCs were infected with LV-NC or LV-ZIP12. After 72 h of infection, cells were serum starved for 24 h in 0.2% FBS/DMEM-F12, and then pretreated with 10 μM LY294002 or 10 μM U0126 for 30 min prior to restimulation with 10% FBS/DMEM-F12 for 48 h. A Cell proliferation was determined by EdU assay (magnification, ×100; n = 5 independent experiments). B Cell migration was determined by wound healing assay. The wounds were imaged every 24 h (magnification, ×40; n = 5 independent experiments). C, D Representative Western blot and semiquantitative analysis of C PCNA and D cyclin D1 protein in each group (C, n = 7 independent experiments; D, n = 5 independent experiments). Ctrl control, PASMCs pulmonary arterial smooth muscle cells, EdU 5-ethynyl-2′deoxyuridine. The data are expressed as the mean ± standard deviation. Error bars represented standard deviation. *P < 0.05, **P < 0.01