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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Notch1 activation of Jagged1 contributes to differentiation of mesenchymal stem cells into endothelial cells under cigarette smoke extract exposure

Fig. 2

Jagged1 expression is regulated by Notch signaling in mesenchymal stem cells (MSCs). A, B Efficiency of Notch-1 knockdown in MSCs by lentiviral vectors harboring shRNA. MSCs were infected with lentiviral vectors GV248-Notch-1-shRNA1, 2, and 3 targeting Notch-1 or a scrambled shRNA vector (Mock) as detailed in Materials and Methods. Notch-1 protein expression was determined by western blotting assays. Notch-1 protein expression was normalized to β-actin levels. *P < 0.05 compared with non-infected MSCs. C–F The Notch signaling pathway was inhibited by incubation with CSE; N1ICD overexpression increased expression levels of Hey1, HeyL, Hes3, and Hes5, which were decreased after transfection with GV248-Notch-1-shRNA2. G, H CSE treatment downregulated Hey1 and Jagged1 expression levels; N1ICD overexpression rescued the decrease in MSCs, whereas the expression levels of Hey1 and Jagged1 were markedly decreased in GV-248-Notch1-shRNA2 transfected MSCs. *P < 0.05 vs. control group, #P < 0.05 vs. CSE group; CSE, cigarette smoke extract

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BMC Pulmonary Medicine

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