Fig. 6
A SAGEs significantly reduced SHS-induced augmentation of RAGE protein in both WT (p = 0.03) and RAGE TG animals (p = 0.02) to near baseline and RAGE expression was not detected in RAGE KO mice. Blots were cropped and densitometrically normalized to β-actin and ratios of RAGE/β-actin are presented. B SAGEs decreased total BALF protein levels (B, p = 0.03–0.04 as indicated) and total BALF cellularity (C, p = 0.01–0.04 as indicated) in WT and RAGE TG exposed to SHS. Total BALF protein and leukocytes were unchanged in RAGE KO mice regardless of exposure (n = 6 mice per group). D-E, Exposure of WT mice to SAGEs prevented SHS-induced decreases in FVC (D, p = 0.01–0.03 as indicated) and PEV at PEF (E, p = 0.02–0.03 as indicated). FEV0.1 was significantly decreased following SHS exposure of WT mice and addition of SAGEs to exposed WT mice elicited an increase in FEV0.1 (F, p = 0.02–0.04 as indicated). Evaluations were obtained via the use of the FlexiVent Instrument (n = 6 animals per group)