Fig. 2

Inactivation of SIK2 inhibited the fibroblast differentiation and TGF-β1 induced pro-fibrotic mediator expression in HFLs. HFLs or SIK2 overexpressed HFLs (HFLs-SIK2) were treated with DMSO (0.1%), ARN-3236 (0.5 μM), 666-15 (0.5 μM) or transfected with SIK2 SiRNA (100 nM). Cells were then treated with TGF-β1 (5 ng/mL) for 12 h. Inactivation of SIK2 inhibited A mRNA expression of α-SMA, COL1A and fibronectin and B cell proliferation in HFLs. Data are expressed as mean ± SEM of at least 4 independent experiments. ***P < 0.001; **P < 0.01; *P < 0.05