Fig. 3

Inhibition of SIK2 reduced CRTC2 phosphorylation in mice. Male BALB/C mice were anesthetized and received intranasal instillation of BLM (5 mg/kg) or its saline vehicle, followed by receiving administration of ARN-3236 (10, 30 mg/kg) or its vehicle. A Representative western blot bands and quantitation of SIK2 and pCRTC2 protein expressions in lungs at day 14 and 28. B Representative western blot bands and quantitation of pCRTC2 protein expressions in lungs at day 28. Data are expressed as mean ± SEM of at least 6 independent experiments. ***P < 0.001; **P < 0.01; *P < 0.05