Fig. 1

Sevoflurane reversed LPS-induced cell pyroptosis and ameliorated ALI in vivo. a H&E staining of mice lung tissues and the histopathological score. b The capillary permeability in mice lung tissue was evaluated by Evans blue staining method. c The wet/dry weight ratio of the lung was calculated for evaluation of pulmonary edema. d The albumin concentration in BALF was measured by ELISA. e, f Macrophages and neutrophils in BALF was measured by a hemocytometer. g Endothelial cell markers (CD31, red) and TUNEL positive cells (TUNEL, green) were observed by immunofluorescence staining. h The pro-inflammatory cytokines (IL-1β, IL-6, IL-18 and TNF-α) in mice lung tissues were measured by RT-qPCR. i The pro-inflammatory cytokines in BALF were measured by ELISA. j, k mRNA levels and protein levels of NLRP3 and cleaved caspase-1 were detected by RT-qPCR and western blot. Con, control group; Se, group treated with sevoflurane; LPS, group treated with LPS; LPS + Se, group co-treated with LPS and sevoflurane. l The proliferation-related factors (CDK2, CDK6, cyclinD1) in mice lung tissues were measured by RT-qPCR. *P < 0.05 compared with control group; ^#P < 0.05 compared with Se group. ^&P < 0.05 compared with LPS group