Fig. 2

Sevoflurane suppressed LPS-induced pyroptotic cell death in MPVECs in vitro. The MPVECs were subjected to LPS treatment, and then intervened with sevoflurane. a, b The cell viability was detected by the MTT assay (a) and trypan blue staining assay (b). c The mRNA levels of NLRP3 and caspase-1 were detected by RT-qPCR analysis. d The protein levels of NLRP3 and cleaved caspase-1 were detected by western blot analysis. e The pyroptotic cells were detected by PI/Hoechst double staining assay. (f) The pro-inflammatory cytokines (IL-1β, and IL-18) in MPVECS and cell supernatant were measured by RT-qPCR and ELISA. Con, control group; Se, group treated with sevoflurane; LPS, group treated with LPS; LPS + Se, group co-treated with LPS and sevoflurane. *P < 0.05 compared with control group; ^#P < 0.05 compared with Se group. ^&P < 0.05 compared with LPS group