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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Sevoflurane anesthesia ameliorates LPS-induced acute lung injury (ALI) by modulating a novel LncRNA LINC00839/miR-223/NLRP3 axis

Fig. 4

Silencing of LINC00839 restrained LPS-induced cell pyroptosis in MPVECs. LINC00839 was silenced in the LPS-treated MPVECs. a, b The cell viability was determined by MTT assay (a) and trypan blue staining assay (b). c The mRNA levels of NLRP3 and caspase-1 were detected by RT-qPCR analysis. d The protein levels of NLRP3 and cleaved caspase-1 were detected by western blot analysis. e The levels of IL-1β, and IL-18 in MPVECS and cell supernatant were measured by RT-qPCR and ELISA. Con, control group; KD-LINC, group with LINC00839 knockdown; LPS, group treated with LPS; LPS + KD-LINC, group with knockdown of LINC00839 on the basis of LPS treatment. *P < 0.05 compared with control group; #P < 0.05 compared with KD-LINC group. &P < 0.05 compared with LPS group

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BMC Pulmonary Medicine

ISSN: 1471-2466

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