Fig. 5

LINC00839 regulated NLRP3/cleaved caspase-1 pathway through miR-223. a The target relationship between LINC00839 and miR-223 was predicted on the starbase website. b The targeting sites in LINC00839 and miR-223 were validated by dual-luciferase reporter gene system assay. c The binding relationship was verified by RNA pull-down assay. LINC00839 and miR-223 were silenced in MPVECS: d The mRNA expression of NLRP3 and caspase-1 was measured by RT-qPCR. e The protein levels of NLRP3 and cleaved caspase-1 were detected by western blot analysis. Wt, wild-type; mut, mutant. Con, control group; KD-LINC, group with LINC00839 knockdown; KD-LINC + KD-miR, group with knockdown of LINC00839 and miR-223. *P < 0.05 compared with control group; ^#P < 0.05 compared with KD-LINC group