Fig. 6

Sevoflurane attenuated LPS-induced cell death and inflammation in MPVECs via the miR-223/NLRP3 axis. The MPVECs were treated with overexpression of NLRP3 or knockdown of miR-223 on the basis of LPS and sevoflurane treatment. a, b The cell viability was detected by the MTT assay (a) and trypan blue staining assay (b). c The pyroptotic cells were detected by PI/Hoechst double staining assay. d The levels of IL-1β, and IL-18 in MPVECS and cell supernatant were measured by RT-qPCR and ELISA. LPS, group treated with LPS; LPS + Se, group co-treated with LPS and sevoflurane. LPS + Se + OE-NLRP3, group with overexpression of NLRP3 on the basis of LPS and sevoflurane treatment. LPS + Se + KD-miR, group with knockdown of miR-223 on the basis of LPS and sevoflurane treatment. *P < 0.05 compared with LPS group; ^#P < 0.05 compared with LPS + Se group