Table 1 Primer sequences used for qRT-PCR analysis of miR-20a-5p, miR-17-5p, miR-93-5p, miR-665 and miR-3202 expression
miRNA | Primer sequence (5'–3') |
|---|---|
hsa-miR-20a-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCT |
JH-hsa-miR-20a-5p-F | GCGCGCTAAAGTGCTTATAGTGC |
hsa-miR-17-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCT |
JH-hsa-miR-17-5p-F | GCCAAAGTGCTTACAGTGC |
hsa-miR-93-5p-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTACCT |
JH-hsa-miR-93-5p-F | GCCAAAGTGCTGTTCGTGC |
hsa-miR-665-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGGGC |
JH-hsa-miR-665-F | GCGCACCAGGAGGCTGAG |
hsa-miR-3202-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATTAAA |
JH-hsa-miR-3202-F | GCTGGAAGGGAGAAGAGC |
U6-hF | CTCGCTTCGGCAGCACA |
U6-hR | AACGCTTCACGAATTTGCGT |
Universal downstream primer | GTGCAGGGTCCGAGGT |